How to use the DIY Kit

Your Kit Includes

Silver Nitrate

Sodium Thiosulfate

Yucca Extract. 


Tools and Materials You Will Need

Inculuded

  • Part A Powder/Crystals - Black Bag
  • Part B Clear Crystals - Clear Bag
  • Part C  Yellow Powder - Yucca Extract - Clear bag

DIY Materials Needed

  • Distilled or Reverse Osmosis Water
  • 2 8oz Brown Glass Bottles (Two beer cups that are clean will work)
  • 32oz Brown Glass Bottle w/Black Sprayer Top(LIght proof Sprayer, or store in dark when not in use)
  • 33 Gallon Black Trash Bag
  • Tape
  • 60 cc/ml Syringe
  • A Cool Dark Place for Storage (Fridge)
  • Nitrile Gloves(please use these)
  • 500ml/2 Cup Glass Mixing Container
  • Small wire whisk
  • Safety Goggles

Basic Safety Recommendations
The following steps require careful measuring, along with basic chemical safety. It is strongly recommended you work on a flat surface with a plastic sheet or several layers of newspaper to protect it. Silver nitrate does stain brown to black, so gloves and safety goggles are recommended.
Sodium thiosulfate has a very low toxicity, make sure you still wash up any spills well to remove all concern. Both of these solutions can be safely rinsed down the drain, when followed by a large amount of water.

Measuring and Mixing Stock Solution "A"

  • Fill your 500ml measuring cup with 125ml of distilled water
  • Mix the black bag's contents into the 125ml of distilled water
  • Pour the solution into a brown glass bottle and seal tightly
  • Label the bottle "A" 
  • Thoroughly clean the 500ml container and rinse with distilled water

Measuring and Mixing Stock Solution "B"

  • Fill the 500ml container with 125ml of distilled water
  • Mix the white crystals from one of the clear bags with the 125ml of distilled water
  • Pour into the brown glass bottle and seal it tightly
  • Label the bottle "B" Sodium Thiosulfate
  • Thoroughly clean the 500ml container and rinse with distilled water

Keep Both "A" and "B" Solutions Cool and Dark! Preferably in the Fridge.

Mixing and Diluting STS
The next step is to mix the two stock solutions together properly, creating the actual Silver Thiosulfate Solution (STS).
Before using the STS, it must be diluted to a weak solution for spraying!

  • Measure 50ml of stock solution "B" with the 60cc syringe and gently squirt it in the 500ml glass measure
  • Thoroughly rinse the syringe with distilled water, or change to a new syringe
  • Measure 50ml of stock solution "A" with the 60cc syringe
  • While whisking solution "B" in the glass measure, slowly squirt solution "A" into the glass measure in less than 30 seconds
  • Pour the mixed solution into the 32oz brown glass bottle with black sprayer top
  • Add 900ml of distilled water to fill the sprayer the rest of the way, leaving room for a little bit of surfactant
  • Add yucca extract in small amounts and shake well
  • Test spray on a leaf to if solution still beads up and rolls off
  • Add additional yucca extract and shake well, until the solution spreads across the leaf instead of beading up - YUCCA EXTRACT SHORTENS STORAGE LIFE - SUNLIGHT DISH SOAP IS AN ALTERNATIVE.
  • Thoroughly rinse all tools using more rinse water than usual

You now have a sprayer bottle with a 1:9 dilution of STS and water. You want to do your best to protect it from light and heat. 

 

Successfully Using STS!
I've spent a great deal of time researching the best method(s) to use for success with STS. First I'm going to detail the steps I've used successfully myself. I'm also including the thoughts and tips I've picked up from other growers. Cannabis is a nearly infinitely variable plant, so it's best if you have information from as many sources as possible. Who knows which tip will specifically fit your plants and setup. 
Regardless of the spraying schedule you use, be sure to thoroughly saturate the branch(es) and beginning flower clusters.

Spraying Schedule I've Used Successfully

  • Spray branch(es) you want reversed 5 days before flipping to flower
  • Spray every 5 days until major male flower formation has begun

Alternative Spraying Schedule A

  • Spray branch(es) you want reversed 2 weeks before flower
  • Spray branch(es) you want reversed on the day you flip to flower

Alternative Spraying Schedule B

  • Spray branch(es) you want reversed on the day you flip to flower

A Note on STS "Burning" the Plant
Many growers have reported the STS solution 'burning' the plant. I strongly recommend diluting your spray further than 1:9, if you experience any burning of your plants.

Additional Tips and Tricks
As always, the more information you have, the better you can potentially achieve. The following are little things which can make a big difference in your results.

Using Clones
When possible, I've found it immensely helpful to work with clones of the same plant, reversing one or more for pollen in a small flowering area first. The pollen is collected and stored. At this point, the pollen can be used on any female plants, including clones of itself.
Though it does involve a second step of collecting and preserving the pollen until use, it allows you to create seeds without dealing with a reversed plant in the room. An additional up side is the ability to store the pollen for years, when preserved properly.

Pollen Control
I STRONGLY recommend using filtered intakes and outtakes on any flowering environment you're creating or using pollen in. Many growers have reported developing allergies to cannabis pollen, after working with it for years on end. Filtering the air going in and out of your flowering rooms keeps pollen contained. (mostly lol)

I use spray bottles of r/o filtered water and spray down the air in the room, any time I didsturb pollen. Knock the pollen out of the air after every session of working with pollen. Be sure to spray down the insides of tents and flower rooms after each run. This will minimize cross contamination from different pollen sources.

Diluting Your Pollen
Any visible bit of pollen you can see is made up of millions of bits of pollen, making one pollen per pistil pollination impossible. Mixing your pollen at one part pollen to 50 parts corn starch or flour prevents waste. Each pistil requires only one bit of pollen to create a seed and the dilution makes this easy.

Using a Makeup Brush to Pollinate With
Many growers report excellent results with evenly pollinating plants, when they use a clean makeup brush. The brush allows easy application of the pollen evenly across the plant. When the pollen is diluted with flour, you're easily able to see which flowers you've already pollinated.

Storing Your Pollen for Years (Possibly 10+ Years)
Pollen can be stored for a long time in a freezer, as long as it has been thorougly dried. Silica packs do not absorb down to a low enough relative humidity, so dried rice is used.

Heat un-cooked rice in an oven at 150F-170F for at least 12hrs. Pour the hot rice into a sealable glass container (I personally use large peanut butter jars). Seal the jar and let it cool to room temperature.

Collect your pollen and carefully transfer it to a foil baking cup or other small container which fits in the jar. Seal the pollen in the rice container and set it in a cool, dark place for 7 days.

Toast some flour in a skillet on the stove until it is a dark tan color, then seal hot in another glass, airtight container and let cool to room temperature. This will remove the moisture from the flour.

Remove the pollen from the dried rice jar and mix it at a ratio of 1 part pollen to 50 parts flour.

Seal the mixed pollen in small containers, foil or plastic sealed pouches or (preferred) 1.5ml centrifuge tubes.

LABEL your containers carefully, using the specific plant name, date and any other information you believe you'll remember later. (Because there's a good chance you won't)

Seal the containers in a thermos and keep it in your freezer. The thermos acts as an additional thermal protection barrier, just in case you have a freezer issue years down the line. It also makes transferring pollen from one place to another a safe operation. Just be sure to keep the thermos at freezing temps, and to limit the amount of time outside the freezer.

Stubborn Female Refusing to Turn?
While the most stable female plants are the type of plants we strongly want to breed with, they are often difficult to get quality amounts of pollen from. These tips should help even the most stubborn female in your stable flip large amounts of viable pollen.

Test Different Spray Schedules
Flower out small clones of the plant you want to reverse. Use a different spray schedule with each clone, keeping careful track. You may find one schedule works much better than the others.

Use Colloidal Silver
Some growers have reported success with the alternative spray schedules, with a follow up spraying of a high ppm colloidal silver solution, 2-3 weeks into flower.

Collect, Dry and Crush the Pods
Have a plant growing great big clusters of male flowers which don't seem to open, or don't have much pollen when they do open?

When the pods have swelled and look like they are fully mature and should be opening, cut them off the stem. Wrap the clusters in paper towels and bury them in a sealed jar of dried rice. (Heat un-cooked rice in an oven at 150-170F for at least 12hrs and cool in a sealed jar) The rice will absorb the moisture from the pods and dry them completely.

When the pods are completely dry and crispy, gently crush them through a tea strainer, or similar fine mesh strainer. You'll find large quantities of viable pollen.

Old Spray (9+ Months)
When the sprayer bottle is properly protected from light, and kept in a cool place, the mixed STS can remain viable for months. You can check to see if the solution will most likely work by pouring a bit out in a clear glass. The solution should be crystal clear.

Any darkening of the solution means at least some of the silver has tarnished. Only use clear solution for best results. Here is a picture of a plant I reversed using STS which had been mixed and stored in my fridge for over 9 months. You can see it worked, but I believe the dish soap (instead of yucca) I added stressed the plant.

MAKES 2 LITRES!